biotinylated rat anti mouse cd8a  (R&D Systems)

Journal: Molecular Therapy

Article Title: Adoptive Transfer of mRNA-Transfected T Cells Redirected against Diabetogenic CD8 T Cells Can Prevent Diabetes

doi: 10.1016/j.ymthe.2016.12.007

Figure Lengend Snippet: CD8 T Cell-Targeting Genes Are Functionally Expressed in a Reporter T Cell Hybridoma following mRNA Transfection

Article Snippet: Biotinylated rat anti-mouse CD8a (clone 53-6.7) and rat anti-mouse B220 (clone RA3-6B2) were from BD Pharmingen and biotinylated mouse anti-insulin antibody (ICBTACLS) was from eBioscience.

Techniques: Transfection

Journal: Molecular Therapy

Article Title: Adoptive Transfer of mRNA-Transfected T Cells Redirected against Diabetogenic CD8 T Cells Can Prevent Diabetes

doi: 10.1016/j.ymthe.2016.12.007

Figure Lengend Snippet: Peptide/β2m/CD3-ζ mRNA Redirects NOD CD8 T Cells to Kill Insulin Reactive CHIB2 T-Hybridoma Cells and Insulin Peptide-Specific CD8 T Cells

Article Snippet: Biotinylated rat anti-mouse CD8a (clone 53-6.7) and rat anti-mouse B220 (clone RA3-6B2) were from BD Pharmingen and biotinylated mouse anti-insulin antibody (ICBTACLS) was from eBioscience.

Techniques:

Journal: Blood

Article Title: Anti-CD45 radioimmunotherapy without TBI before transplantation facilitates persistent haploidentical donor engraftment

doi: 10.1182/blood-2014-12-617019

Figure Lengend Snippet: Chimerism following haploidentical BMT with a conditioning regimen of 90Y-anti-CD45 RIT and FLU. (A) Mean CD8+ donor chimerism in peripheral blood 28 days after haploidentical BMT in mice given FLU × 5 days (100 mg/kg per day IP on days −8 to −4) and 200, 300, or 400 µCi 90Y-DOTA-30F11 on day −3 followed by 1.5 × 107 haploidentical BM cells on day 0. (B) MFC analysis of peripheral blood 28 days after haploidentical BMT in a B6SJLF1/J transplanted mouse pretreated with FLU 100 mg/kg per day × 5 days and 200 µCi 90Y-DOTA-30F11, depicting the percentage of CD8+ cells expressing the H-2Dd haplotype.

Article Snippet: Cells were then stained with CD8 + -specific biotinylated rat anti-mouse CD8a (clone 53-6.7), followed by PerCP-streptavidin and/or phycoerythrin-R–conjugated mouse anti-mouse H-2D d , all from BD Pharmingen.

Techniques: Expressing

Journal: Blood

Article Title: Anti-CD45 radioimmunotherapy without TBI before transplantation facilitates persistent haploidentical donor engraftment

doi: 10.1182/blood-2014-12-617019

Figure Lengend Snippet: Chimerism following haploidentical BMT with a conditioning regimen of 90Y-anti-CD45 RIT without FLU. (A) Mean CD8+ donor chimerism in peripheral blood 28 days after haploidentical BMT in mice pretreated with 200, 300, or 400 µCi 90Y-DOTA-30F11 on day −3, without FLU. MFC analysis of peripheral blood 28 days after haploidentical BMT in a B6SJLF1/J mouse pretreated with (B) FLU × 5 days (100 mg/kg per day IP on days −8 to −4) and 400 µCi 90Y-DOTA-30F11 on day −3 or (C) FLU × 5 days without anti-CD45 RIT, both transplanted with 1.5 × 107 haploidentical BM cells on day 0, depicting the percentage of CD8+ cells expressing the H-2Dd haplotype.

Article Snippet: Cells were then stained with CD8 + -specific biotinylated rat anti-mouse CD8a (clone 53-6.7), followed by PerCP-streptavidin and/or phycoerythrin-R–conjugated mouse anti-mouse H-2D d , all from BD Pharmingen.

Techniques: Expressing

Journal: Blood

Article Title: Anti-CD45 radioimmunotherapy without TBI before transplantation facilitates persistent haploidentical donor engraftment

doi: 10.1182/blood-2014-12-617019

Figure Lengend Snippet: Chimerism following haploidentical BMT with a conditioning regimen of 90Y-anti-CD45 RIT with pre- and post-BMT CY. MFC analysis of peripheral blood 28 days after haploidentical BMT in a B6SJLF1/J transplanted mouse that received 1.5 × 107 haploidentical BM cells on day 0 and (A) 300 µCi 90Y-DOTA-30F11 on day −3 and CY (200 mg/kg per day on days −2 and +2) or (B) CY alone without RIT, depicting the percentage of CD8+ cells expressing the H-2Dd haplotype.

Article Snippet: Cells were then stained with CD8 + -specific biotinylated rat anti-mouse CD8a (clone 53-6.7), followed by PerCP-streptavidin and/or phycoerythrin-R–conjugated mouse anti-mouse H-2D d , all from BD Pharmingen.

Techniques: Expressing

Journal: Blood

Article Title: Anti-CD45 radioimmunotherapy without TBI before transplantation facilitates persistent haploidentical donor engraftment

doi: 10.1182/blood-2014-12-617019

Figure Lengend Snippet: Chimerism following haploidentical BMT with a conditioning regimen of 90Y-anti-CD45 RIT, FLU, and/or CY. Mean percentage of CD8+ cells expressing the H-2Dd haplotype in peripheral blood from transplanted mice 28 days after BMT. Recipient mice were treated with 300 µCi 90Y-DOTA-30F11 on day −3 alone, with FLU × 5 days (100 mg/kg per day on days −8 to −4), with CY (200 mg/kg per day on days −2 and +2), or with both FLU and CY.

Article Snippet: Cells were then stained with CD8 + -specific biotinylated rat anti-mouse CD8a (clone 53-6.7), followed by PerCP-streptavidin and/or phycoerythrin-R–conjugated mouse anti-mouse H-2D d , all from BD Pharmingen.

Techniques: Expressing

Journal: Blood

Article Title: Anti-CD45 radioimmunotherapy without TBI before transplantation facilitates persistent haploidentical donor engraftment

doi: 10.1182/blood-2014-12-617019

Figure Lengend Snippet: Donor origin CD8 + cells

Article Snippet: Cells were then stained with CD8 + -specific biotinylated rat anti-mouse CD8a (clone 53-6.7), followed by PerCP-streptavidin and/or phycoerythrin-R–conjugated mouse anti-mouse H-2D d , all from BD Pharmingen.

Techniques:

Journal: PloS one

Article Title: Cross-protective peptide vaccine against influenza A viruses developed in HLA-A*2402 human immunity model.

doi: 10.1371/journal.pone.0024626

Figure Lengend Snippet: Figure 4. An accumulation of murine CD3+ and CD8+ cells around the bronchioles in intranasally immunized mice. A24Tg mice were immunized three times at 7 to 9 days intervals i.n.(A,C,D,E) or s.c.(B) with PA130–138, PB1430–438 and PB2549–557 peptides in the presence of CpG-ODN (B,C,D), Tyrosinase206–214 plus CpG-ODN (E) or CpG-ODN plus empty-liposome solution (A). Lungs were harvested at day 7 after the final immunization, embedded in O.C.T. compound, frozen in dry ice-2-propanol. Ten mm thick frozen sections were prepared. The sections were post- fixed in acetone:ethanol (1:1) solution and blocked endogenous avidin and biotin activity, then stained with anti-mouse CD3 (A,B,C) or anti-mouse CD8a (D,E). doi:10.1371/journal.pone.0024626.g004

Article Snippet: The sections were stained with biotinylated hamster anti-mouse CD3 (eBioscience, San Diego, CA) or biotinylated rat anti-mouse CD8a (R&D Systems ) antibodies.

Techniques: Avidin-Biotin Assay, Activity Assay, Staining